Multi Resistant strains were according to the Center for Disease prevention and Control. MDR bacteria are defined as resistant to at least three different classes of antibiotics. The diameter of the inhibition zone formed around the disc was measured and compared to the critical values of each antibiotic disc (according to CLSI) to qualify the target bacteria as sensitive or resistant. Different families of antibiotics (discs obtained from OXOID) were used in this study included Cephems Beta-lactam + inhibitor, Cephalosporines (1st, 2nd and 3rd generations) Fluoquinolones Tetracyclins Folate pathway inhibitors Nitrofurans Aminoglycosides Monobactams and Carbapenemes. Antimicrobial Susceptibility TestingĪntibiotic susceptibility was determined using the disc diffusion method on Mueller Hinton agar according to the Guidelines of the Clinical Laboratory Standards Institute (GCLSI). The isolated bacteria were then identified by using Gram Stain and their biochemical characteristics using Remelrap ID system kits.Ģ.3. coli colonies were colorless on McConkey agar and colorless to blue on CLED agar. The determination of lactose non-fermenter strains was using by observation colonies on MacConkey agar and Cystine Lactose Electrolyte Deficient (CLED) Agar. Additionally, the 0.01 mL of urine sample was inoculated on CHROME Orientation agar (HyLabs Ltd) by spread plate technique. Test yielding ≥ 10 5 CFU/ml are regarded as Positive. The tube was incubated 18 - 24 hours at 37˚C. The paddle was transferred into the tube and cap was screwed back loosely, to allow for free transfer of atmosphere. The Uritest paddle was dipped into the aseptically collected urine sample and removed immediately. This prospective study was carried out to assess the sensitivity and resistance pattern of lactose non-fermenting Escherichia coli from July 2018 to December 2018 in the Laboratory of Microbiology at Luanda Medical Center, Angola.īacteria were isolated according to the routine protocol of urinalysis using MacConkey/CLED HY-Uritest and chromogenic media (Hy-Labs, Israel). The aim of this study was conducted to determine the antibiotic resistance pattern of lactose non-fermenting Escherichia coli. According to the Antimicrobial Resistance Global Report of WHO, the data about antibiotic resistance obtained from the African countries is still not enough. coli as the etiological agent of urinary tract infection, while the Gram-negative, non-fermenting multi-resistant bacilli make the treatment of these organisms very difficult and expensive. Now there is not a lot of data about lactose non-fermenting E. coli has a number of virulence factors and exhibit antibiotic resistance. coli can be “atypical” non-lactose fermenting. Historically been reported that up to 10% of E. coli is facultative anaerobic, Gram-negative bacilli ferments lactose to produce hydrogen sulphide. coli is the most common and predominated bacteria causing UTIs in human beings. This is especially true for the developing countries including African continent where self-medication, overuse and misuse of antibiotics leads to the emergence of multi drug resistant (MDR)bacteria. The level of bacterial resistance to antibiotics is growing from year to year and is becoming one of the main problems in the world. The Urinary tract infections (UTIs) are serious health affecting problems worldwide.
0 Comments
Leave a Reply. |
AuthorWrite something about yourself. No need to be fancy, just an overview. ArchivesCategories |